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Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit

  • Cat.No.:E-BC-K071-M

  • Detection instrument: Microplate reader(510-530 nm,optimum wavelength: 520 nm)

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Price: $220

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Detection principle

The excess iron is added to the serum to bind all the ferritin in the serum, and the excess iron is adsorbed by adding the iron adsorbent. The iron bind with the ferritin is separated from the protein by the action of acid solution and reductant. Fe3+ in serum is reduced to Fe2+, Fe2+ binds with bipyridine to form pink complex. In a certain range, the amount of TIBC is positively correlated with the depth of color. The iron content measured is, minus serum iron value, which is called unsaturated iron binding force. Total iron binding capacity minus serum iron value is unsaturated iron binding capacity (UIBC).

Performance characteristics

Synonyms TIBC
Sample type Serum
Sensitivity 0.14 mg/L
Detection range 0.31-50 mg/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 2.3%Average intra-assay CV: 1.5%
Other instruments required Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-50 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Rat serum


Porcine serum


Rabbit serum


Chicken serum


Machin serum


Note: The diluent is double distilled water.


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