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Bradford Protein Colorimetric Assay Kit

Cat:E-BC-K168-S Citations (2)
Manual MSDS

Price: $ 200

Price: $ 100

Size:
500Assays 100Assays
Quantity:
  • Sample Type: serum;plasma;animal tissue
  • Detection Instrument: Spectrophotometer(595 nm)
  • Reasearch Areas: Amino acids proteins;Liver Renal function biomarkers
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Product Details
Detection Principle Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Synonyms TP
Sample Type serum,plasma,animal tissue
Sensitivity 0.026 mg/mL
Detection Range 0.026-1.2 mg/mL
Detection Method Colorimetric method
Assay Time 50 min
Precision inter-assay CV: 2.3% | intra-assay CV: 1.8%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 40-60
10% Mouse liver tissue homogenization 8-12
Rat serum 40-60
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.