Bradford Protein Colorimetric Assay Kit
Price: $ 200
Price: $ 100
Size:
500Assays
100Assays
Quantity:
- Sample Type: serum;plasma;animal tissue
- Detection Instrument: Spectrophotometer(595 nm)
- Reasearch Areas: Amino acids proteins;Liver Renal function biomarkers
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Product Details
Detection Principle | Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm. | ||||||||
Synonyms | TP | ||||||||
Sample Type | serum,plasma,animal tissue | ||||||||
Sensitivity | 0.026 mg/mL | ||||||||
Detection Range | 0.026-1.2 mg/mL | ||||||||
Detection Method | Colorimetric method | ||||||||
Assay Time | 50 min | ||||||||
Precision | inter-assay CV: 2.3% | intra-assay CV: 1.8% | ||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||
Storage | This product can be stored at 2~8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. | ||||||||
Valid Period | 12 months | ||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
Citations
-
Visual Detection of Clostridium perfringens Alpha Toxin by Combining Nanometer Microspheres with Smart Phones
IF:4.152
Journal:Microorganisms
DOI:10.3390/microorganisms8121865
PMID:33256026
-
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress
IF:1.628
Journal:Journal of Oleo Science
PMID:35965085