TP(Thiamphenicol) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E084

      • 96T
      - +
      Price: $276

      sample_type: Tissue,Egg,Milk

      Sensitivity: 1 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Thiamphenicol (TP) in samples, such as tissues, eggs, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, TP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-TP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of TP. The concentration of TP in the samples can be calculated by comparing the OD of the samples to the standard curve.

      Technical indicator

      Sensitivity: 0.5 ppb (ng/mL)

      Reaction mode: 25℃,30 min~30 min~15 min

      Detection limit: Tissue, Eggs ---1 ppb; Raw milk---0.3 ppb                                         

      Cross-reactivity: Thiamphenicol---100%,Florfenicol---71%,Florfenicol Amine---7%

      Sample recovery rate: Tissue---90%±30%, Raw milk---100%±30%

      Kits components



      ELISA Microtiter Plate

      96 wells

      Standard Liquid

      1.5 mL each

      (0 ppb, 0.5ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb)

      HRP Conjugate

      12 mL

      Antibody Working Solution

      7 mL

      Substrate Reagent A

      7 mL

      Substrate Reagent B

      7 mL

      Stop Solution

      7 mL

      20×Concentrated Sample Solution

      25 mL

      20×Concentrated Wash Buffer

      25 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece


      1 copy

      Other supplies required

      Instrument: Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01g). Nitrogen Evaporators, Water bath.

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents: Ethyl Acetate, N-hexane 

      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.

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      MSDS for ELISA ISO 9001

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