VERO C1008 [Vero E6] cell line

Name: VERO C1008 [Vero E6] cell line
Brand: Elabscience
SKU: EP-CL-0491
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Cell Name	VERO C1008 [Vero E6] cell line
Organism	Cercopithecus aethiops
Age	adult
Tissue	Normal Kidney
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	This cell line was established in 1979, cloned from VERO 76 into microtiter plates by using the dilution method .
Biosafety Level	1

Complete Growth Medium	MEM(EP-CM-L0191)+10% FBS+1% P/S(EP-CM-L0048) (VERO C1008 [Vero E6] cell line complete medium (EP-ML-0491) is recommended.)
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1: 2-1: 4
Medium Renewal	2-3 times per week
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

EP-CM-P0139	MEM (with NEAA), powder
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043	0.25% Trypsin Solution

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