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Catalog number:EP-CL-0491
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Add to cart Compare Bulk requestCell Name | VERO C1008 [Vero E6] cell line |
Organism | Cercopithecus aethiops |
Age | adult |
Tissue | Normal Kidney |
Morphology | Epithelial |
Growth Properties | Adherent |
Descriptions | This cell line was established in 1979, cloned from VERO 76 into microtiter plates by using the dilution method . |
Biosafety Level | 1 |
Complete Growth Medium | DMEM+10% FBS+1% P/S |
Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
Subcultivation Ratio | 1: 2-1: 4 |
Medium Renewal | 2-3 times per week |
Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Recommendations for handling of cryopreserved cells