Zinc (Zn) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K137-A

      Size:
      • R1:50 mL×1, R2:5 mL×1
      Qty:
      - +
      Price: $240

      Detection method: Colorimetric method

      Detection instrument: Biochemistry analyzer

      Valid period: 12 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Application

      This kit is used for in vitro quantitative determination of zinc ion (Zn2+) in serum, plasma, urine, etc.

       

      Detection principle

      The zinc ion in the sample forms blue complex with zinc reagent at room temperature. Measure the absorption value at 630 nm and calculate the content of zinc. 

      Experimental instrument

      automatic biochemical analyzer or semi-automatic biochemical analyzer

       

      Sample preparation

      The kit is suitable for detecting serum, heparin anticoagulated plasma, urine and semen. Avoid hemolysis. 


      Operation steps

      1) Main performance parameters

      Dominant wavelength

      630 nm

      Optical path

      1 cm

      Reaction method

      Endpoint method

      Reaction temperature

      37°C

      Reaction direction

      Up

       

       

       

      2) Operation table

      Sample

      25 μL

      Reagent 1

      250 μL

      Reagent 2

      25 μL

      Mix fully and incubate for 5 min at 37°C, measure the absorbance A.

      Technical parameter

      1.  Absorbance for the blank control: A630 (1.0 cm)0.2.

      2. Detection range: 0~30 µmol/L, r20.995.

      3. Accuracy: relative deviation15.0%.

      4. Precision: the intra-assay CV4.0% and the inter-assay CV6.0%.

      5. Sensitivity: for 15.4 µmol/L sample, the absorbance is 0.1-0.2.


      Notes

      1. The kit is for scientific research only. 

      2. Instructions should be followed strictly, changes of operation may result in unreliable results. 

      3. The validity of kit is 12 months.

      4. Do not use components from different batches of kit.

      5. Do not use hemolytic serum samples. 

      6. As the concentration of Zn2+ in serum is very low, avoid zinc contamination of vessels and reagents used in the experiment.

      7. The ratio of sample and reagent can be scaled as required.

      8. The sample needs to be diluted with deionized water before determination once the concentration is beyond the linear range. The result should be multiplied by the dilution factor.

      9. Choose a filter with the closest wavelength to the required one if there is no suitable filter at hand.

      10. No sample deproteinization and sample blanks is required for this method.

      11. If the human body surface such as skin, eyes, etc., is contaminated by the reagent, it must be rinsed with clean water. And in case of accidental ingestion, seek medical treatment.

      12. The product does not contain poisonous or biological hazardous material.

      • Show all (2)
      • Reviews (0)
      • Q&A (2)
      Q

      K****LSubmitted [ Jul 26 2018 ]

      Asked: Whats the component present in the R2

      Reply

      A

      adminSubmitted [ Jul 26 2018 ]

      Answered: Dear KALLOL, Thanks for your question. Here is the component present in the R2: 2-(5-Bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl) amino]phenol, disodium salt, dihydrate(5-Br-PAPS). Please feel free to contact us if any question. Thank you.

      Q

      s*********tSubmitted [ Jul 18 2018 ]

      Asked: What are the components involved in ZINC Reagent (R1) ?

      Reply

      A

      adminSubmitted [ Jul 18 2018 ]

      Answered: Dear sangeetha t, Thanks for your question. The Reagent 1 (R1) is Tris buffer with the concentration of 100 mmol/L. Please feel free to contact us if any question.

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