Angiotensin Converting Enzyme (ACE1) Activity Assay Kit (E-BC-K003-S)
For research use only.
| Detection Principle | N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG) have the maximum absorption peak at 340 nm, angiotensin converting enzyme catalyze N-(3[2-Furyl ]Acryloyl)-Phe-Gly-Gly to produce FAP and GG, and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm. |
| Synonyms | ACE1 |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (340 nm) |
| Research Area | Amino Acids And Proteins , Metabolic Diseases |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 27.5 U/L |
| Detection Range | 27.5-682 U/L |
| Precision | inter-assay CV: 4.4% | intra-assay CV: 0.03 |
| Sample Volume | 100 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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