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For research use only.
| Verified Samples |
Verified Samples in WB: various cell lines Verified Samples in IHC: Rat kidney, Human colon carcinoma, Mouse liver Verified Samples in IF: NIH/3T3, C6 |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200 |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human ASB3 |
| Synonyms | Ankyrin repeat and SOCS box containing 3,Ankyrin repeat and SOCS box containing protein 3,Ankyrin repeat and SOCS box protein 3,ASB 3,ASB-3,ASB3,ASB3,FLJ10123,FLJ10421,MGC12531,MGC132002,MGC996,OTTHUMP00000200662,Weakly similar to ankyrin brain variant 2 |
| Swissprot | |
| Observed MW |
58 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2011] |
Other Clones
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