Cysteine (Cys) Colorimetric Assay Kit (E-BC-K352-M)
For research use only.
| Detection Principle | Phosphotungstic acid can be reduced by Cys and form tungsten blue, which has an absorption peak at 600 nm. Cys content can be calculated with the absorbance at 600 nm. |
| Synonyms | Cys |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (600-620 nm, optimum wavelength: 600 nm) |
| Research Area | Amino Acids And Proteins , Ferroptosis |
| Other Reagents Required | Phosphoric acid |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 mmol/L |
| Detection Range | 0.07-2.0 mmol/L |
| Precision | inter-assay CV: 1.3% | intra-assay CV: 1.1% |
| Sample Volume | 50 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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