Glutamic Acid (Glu) Colorimetric Assay Kit (E-BC-K903-M)
For research use only.
| Detection Principle | Glutamic acid can reduce NAD+ to NADH. NADH, under the action of hydrogen transmitter, reduces WST-8 to form yellow product, which has a characteristic absorption peak at 450 nm. Glutamic acid content can be calculated by measuring the OD value at 450 nm. |
| Synonyms | Glu |
| Sample Type | Serum,Plasma,Urine,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm,optimum wavelength: 450 nm) |
| Research Area | Amino Acids And Proteins , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl). |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.006 mmol/L |
| Detection Range | 0.006-0.8 mmol/L |
| Precision | inter-assay CV: 4.4% | intra-assay CV: 0.8% |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1-2 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat pancreatic tissue homogenate | 1 |
| 10% Bovine liver tissue homogenate | 1 |
| 10% Porcine heart tissue homogenate | 1 |
| Human serum | 3-5 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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