Glutathione Peroxidase 4 (GPX4) Activity Assay Kit (E-BC-K883-M)
For research use only.
| Detection Principle |
Glutathione peroxidase 4 (GPX4) is a kind of glutathione peroxidase containing selenocysteine, which can reduce phospholipid hydroperoxide to phosphatidyl alcohol, thus protecting the cell membrane from oxidative damage. GPX4 catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents.The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system. |
| Synonyms | GPX4 |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Ferroptosis |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.22U/L |
| Detection Range | 3.22-44.69U/L |
| Precision | inter-assay CV: 0.4-3.4% | intra-assay CV: 1.2-3.1% |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 5-50 |
| 10% Mouse kidney tissue homogenate | 2-6 |
| 10% Mouse lung tissue homogenate | 2-5 |
| 2.05×10^6 293T cells | 1 |
| 1.9 × 10^6 4T1 cells | 1 |
| 2.03 × 10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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