Human HGF (Hepatocyte Growth Factor) CLIA Kit (E-CL-H0083)

Name: Human HGF (Hepatocyte Growth Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0083
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human HGF (Hepatocyte Growth Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HGF in samples.No significant cross-reactivity or interference between Human HGF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	52772.000	59776.000	56274.000	56247
1000	23651.000	28145.000	25898.000	25871
500	12358.000	10706.000	11532.000	11505
250	5561.000	5945.000	5753.000	5726
125	2722.000	2672.000	2697.000	2670
62.5	1421.000	1305.000	1363.000	1336
31.25	693.000	695.000	694.000	667
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.980	235.210	773.470	101.170	229.840	801.840
Standard deviation	10.700	25.870	84.850	10.910	20.590	60.780
CV（%）	10.190	11.000	10.970	10.780	8.960	7.580

Recovery

The recovery of Human HGF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-106	100
EDTA plasma （n=8）	86-99	92
Cell culture media （n=8）	91-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HGF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-100	98-114	91-104
1:2	Average	94	104	98
1:4	Range	94-106	91-104	94-106
1:4	Average	100	96	99
1:8	Range	89-99	92-105	101-117
1:8	Average	94	98	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HGF. You can calculate the concentration of Human HGF in the samples by comparing the RLU value of the samples to the standard curve.

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