Hydrogen Peroxide (H2O2) Colorimetric Assay Kit (E-BC-K102-M)
For research use only.
| Detection Principle | Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H2O2 content can be calculated by measuring the absorbance value at 405 nm. |
| Sample Type | Serum,Plasma,Urine,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (402-407 nm, optimum wavelength: 405 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.41 mmol/L |
| Detection Range | 0.41-125 mmol/L |
| Precision | inter-assay CV: 3.6% | intra-assay CV: 3.2% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Porcine serum | 1 |
| Human urine | 1 |
| Cell homogenate | 1 |
| 10% Plant tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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