Lipid Peroxide (LPO) Colorimetric Assay Kit (E-BC-K176-M)
For research use only.
| Detection Principle | With 45°C incubation for 60 min, one molecule of LPO react with two molecule of chromogenic reagent, to produce a stable chromophore which have the maximum absorption peak at 586 nm. The content of LPO in samples can be calculated by standard curve or calculation formula. |
| Synonyms | LPO |
| Sample Type | Serum,Plasma,Urine,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (580-590 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Anhydrous ethanol, PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.70 μmol/L |
| Detection Range | 0.70-80 μmol/L |
| Precision | inter-assay CV: 3.5% | intra-assay CV: 3.1% |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Urine | 1 |
| 10% Rat kidney tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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