Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples) (E-BC-K028-M)
For research use only.
| Detection Principle | MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm. |
| Synonyms | MDA |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-540 nm, optimum wavelength: 532 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Absolute ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.29 nmol/mL |
| Detection Range | 0.29-100 nmol/mL |
| Precision | inter-assay CV: 3.5% | intra-assay CV: 3.3% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| A549 cells | 1 |
| HepG2 cells | 1 |
| 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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