Mouse DEFa5 (Defensin Alpha 5, Paneth Cell Specific) CLIA Kit (E-CL-M0254)

Name: Mouse DEFa5 (Defensin Alpha 5, Paneth Cell Specific) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0254
Price: 180.0 USD
Availability: InStock

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Mouse DEFa5 (Defensin Alpha 5, Paneth Cell Specific) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse DEFa5 in samples.No significant cross-reactivity or interference between Mouse DEFa5 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	31997.000	36665.000	34331.000	34290
10	11803.000	14309.000	13056.000	13015
5	6241.000	5309.000	5775.000	5734
2.5	2491.000	2897.000	2694.000	2653
1.25	1510.000	1340.000	1425.000	1384
0.63	951.000	925.000	938.000	897
0.31	636.000	694.000	665.000	624
0	39.000	43.000	41.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.960	3.180	8.410	1.000	3.380	8.520
Standard deviation	0.090	0.240	0.860	0.090	0.340	0.640
CV（%）	9.380	7.550	10.230	9.000	10.060	7.510

Recovery

The recovery of Mouse DEFa5 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-101	94
EDTA plasma （n=8）	92-106	99
Cell culture media （n=8）	91-104	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse DEFa5 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-105	97-111	89-104
1:2	Average	98	102	96
1:4	Range	84-97	87-101	94-105
1:4	Average	91	92	99
1:8	Range	95-106	94-105	100-116
1:8	Average	101	99	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DEFa5. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DEFa5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DEFa5, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse DEFa5. You can calculate the concentration of Mouse DEFa5 in the samples by comparing the RLU value of the samples to the standard curve.

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