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Porcine sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit (E-EL-P0485)

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All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 4.69 ng/mL
Detection Range 7.81-500 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Porcine
Specificity This kit recognizes Porcine sC5b-9 in samples. No significant cross-reactivity or interference between Porcine sC5b-9 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine sC5b-9. You can calculate the concentration of Porcine sC5b-9 in the samples by comparing the OD of the samples to the standard curve.
sC5b-9 (soluble C5b-9) is a terminal complement complex that is formed as a result of activation of the complement system by classical, lectin, or alternative pathways. Therefore, measuring the sC5b-9 complex can serve as an alternative marker for terminal complement activation. In the current study, we examined the possibility of soluble C5b-9 (sC5b9), the terminal complement complex, as a potential biomarker for TMA development in patients with active GVHD. Traumatic brain injury (TBI) following activation of the terminal complement pathway leads to the formation of the membrane attack complex (MAC无C5b-9), which induces neuronal cell death and host-mediated secondary brain injury. Serum levels of soluble MAC (sC5b-9) have not been determined in isolated TBI patients. Therefore, sC5b-9 as a biomarker in atypical hemolytic uria syndrome (aHUS), due to abnormal activation of the complement pathway at the cell surface level, can be detected by ELISA using plasma samples for sC5b-9, or as a stable MAC pore.
Research Area AHUS
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