Squalene Synthase (SQS) Activity Colorimetric Assay Kit (E-BC-K794-M)
For research use only.
| Detection Principle | Squalene synthase (SQS) is involved in the isoprene biosynthesis pathway, and its catalytic action is the first rate-limiting step in sterol biosynthesis. Squalene synthetase can produce squalene under the co-action of cofactors. The principle of this kit is that the absorbance of cofactors can be measured at 340 nm, and the amount of cofactors consumed in the catalytic reaction is proportional to the activity of squalene synthase, and the activity of squalene synthase can be calculated according to the change of absorbance value per unit time. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.44 U/L |
| Detection Range | 0.44-16.62 U/L |
| Precision | inter-assay CV: 2.1-7.2% | intra-assay CV: 1.9-5.0% |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 1×10^6 Hela 60 cells | 1 |
| Rat plasma | 1 |
| Porcine plasma | 1 |
| Human plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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