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Protein Purification and Separation


For most researchers of biochemistry, structural biology and functional proteomics, they will spent a large amount of time and devote plenty of energy in specific protein or other biomolecules separation and purification. The step above is the most basic experiment procedure and the most important one in whole experimentation which is of crucial significance for laboratory result and experimental cost. Only make it efficiently done could we win the match with other peers.

 

So who is the “clutch player” in this “nonexplosive warfare”? The answer is high-quality Chromatographic Media. Elabscience® offers an extensive range of chromatographic media for most of biomolecules separation and purification. We have collected our best products for all kinds of packing to help you separate and purify your target molecules easily and efficiently. All of these products have been strictly verified by our responsible and talented technicians, thus are guaranteed to be high-quality and can help you to cut cost and get favorable results. View and see how Elabscience® can help you with your biochemical researches.

  • Gel filtration chromatography
  • Affinity chromatography
  • Ion exchange chromatography
  • Hydrophobic interaction chromatography

Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules by their size or molecular weight. SEC can be used for wide ranges of separation and purification of components with large molecular weight differences and low resolution requirement, such as proteins, polysaccharides and other macromolecules.


Cat. No.

Product name

Matrix

Particle size range

Average particle size

E-CM-GF01

Focurose 4B

4% agarose

45-165 µm

90 µm

E-CM-GF02

Focurose 6B

6% agarose

45-165 µm

90 µm

E-CM-GF03

Focurose 4BB

Cross-linked 4% agarose

165-300 µm

200 µm

E-CM-GF04

Focurose 6BB

Cross-linked 6% agarose

165-300 µm

200 µm

E-CM-GF05

Focurose CL-4B

Lowly cross-linked 4% agarose

45-165 µm

90 µm

E-CM-GF06

Focurose CL-6B

Lowly cross-linked 6% agarose

45-165 µm

90 µm

E-CM-GF07

Focurose 4FF

Highly cross-linked 4% agarose

45-165 µm

90 µm

E-CM-GF08

Focurose 6FF

Highly cross-linked 6% agarose

45-165 µm

90 µm

E-CM-GF09

Focudex G-25 Medium

Dextran

45-165 µm

100-300 µm

E-CM-GF10

Focudex G-75 Medium

Cross-linked dextran

45-165 µm

80-300 µm

Affinity chromatography is a powerful chromatography technique of separating and purifying proteins based on the highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand. Such interactions including hydrogen bonding, ionic interaction, disulfide bridges, hydrophobic interaction, etc. Affinity chromatography are widely recognized for their high selectivity, high resolution and high capacity.


Cat. No.

Product name

Matrix

Binding capacity

E-CM-AF03

NHS Focurose 4FF

Highly cross-linked 4% agarose

16-23 μmol /mL (media)

E-CM-AF04

GST Focurose 4FF

Highly cross-linked 4% agarose

15-25mg (GST-tagged protein)/mL (medium)

E-CM-AF05

GST Focurose 4B

4% agarose

15-25mg (GST-tagged protein)/mL (media)

E-CM-AF06

Ni Focurose 6FF (TED)

Highly cross-linked 6% agarose

20-30 mg (His-tagged protein)/mL (medium)

E-CM-AF07

Ni Focurose 6FF (IMAC)

Highly cross-linked 6% agarose

45 mg (His-tagged protein)/mL (medium)

E-CM-AF08

Ni Focurose 6FF (IDA)

Highly cross-linked 4% agarose

40-50 mg (His-tagged protein)/mL (medium)

E-CM-AF09

Co Focurose 6FF (IMAC)

Highly cross-linked 6% agarose

45 mg (His-tagged protein)/mL (media)

E-CM-AF11

Protein A Focurose 4HF

Highly cross-linked 4% agarose and cellulose

~30 mg (Human IgG)/mL (media)

E-CM-AF12

Protein G Focurose 4FF

Highly cross-linked 4% agarose

~25 mg (Human IgG)/mL (media)

E-CM-AF13

Benzamidine Focurose 4FF(HS)

Highly cross-linked 4% agarose

25-50 mg (Trypsase)/mL (media)

E-CM-AF14

EAH Focurose 6FF

Highly cross-linked 6% agarose

0.4-0.5 mM Cl-/mL (media)

E-CM-AF15

Protein A Focurose 6FF

Highly cross-linked 6% agarose

0.4-0.6 mM Cl-/mL (media)

Ion exchange chromatography (IEX) is a practical chromatography process that separates ions and polar molecules based on their respective charged groups. IEX can be used for separation and purification of charged large proteins, small nucleotides, and amino acids.

Cat. No.

Product name

Matrix

Ionic capacity

Binding capacity

E-CM-IE01

SP Focurose 6FF

Highly cross-linked 6% agarose

180-300 µmol H+/mL (media)

100 mg Lysyme /mL (media)

E-CM-IE02

SP Focurose 6HP

Highly cross-linked 6% agarose

200-300 µmol H+/mL (media)

120 mg Lysyme /mL (media)

E-CM-IE03

SP Focurose HPR

Highly cross-linked agarose and cellulose

180-280 µmol H+/mL (media)

100 mg Lysyme /mL (media)

E-CM-IE04

SP Focurose 6HF

Highly cross-linked 6% agarose and cellulose

140-220 µmol H+/mL (media)

120 mg Lysyme /mL (media)

E-CM-IE05

SP Focurose 6XL

Highly cross-linked agarose and dextran

180-250 µmol H+/mL (media)

160 mg Lysyme /mL (media)

E-CM-IE07

CM Focurose 6FF

Highly cross-linked 6% agarose

90-150 µmol H+/mL (media)

110 mg Lysyme /mL (media)

E-CM-IE08

CM Focurose 6HP

Highly cross-linked 6% agarose

90-160 µmol H+/mL (media)

95 mg Lysyme /mL (media)

E-CM-IE09

Q Focurose 6FF

Highly cross-linked 6% agarose

180-360 µmol Cl-/mL (media)

90 mg BSA/mL (media)

E-CM-IE10

Q Focurose 6HP

Highly cross-linked 6% agarose

200-280 µmol Cl-/mL (media)

130 mg BSA/mL (media)

E-CM-IE11

Q Focurose 6HPR

Highly cross-linked agarose and cellulose

180-250 µmol Cl-/mL (media)

120 mg BSA/mL (media)

E-CM-IE12

Q Focurose 6HF

Highly cross-linked 6% agarose and cellulose

160-220 µmol Cl-/mL (media)

100 mg BSA/mL (media)

E-CM-IE13

Q Focurose 6XL

Highly cross-linked agarose and dextran

200-350 µmol Cl-/mL (media)

130 mg BSA/mL (media)

E-CM-IE15

DEAE Focurose 6FF

Highly cross-linked 6% agarose

150-300 µmol Cl-/mL (media)

110 mg BSA/mL (media)

E-CM-IE16

DEAE Focurose 6XL

Highly cross-linked agarose and dextran

460-510 µmol Cl-/mL (media)

180 mg BSA/mL (media)

E-CM-IE17

DEAE Focurose 6HP

Highly cross-linked 6% agarose

180-260 µmol Cl-/mL (media)

90 mg BSA/mL (media)

E-CM-IE18

ANX Focurose 6FF

Highly cross-linked 6% agarose

100-200 µmol Cl-/mL (media)

80 mg BSA/mL (media)

E-CM-IE19

MMA Focurose 6FF

Highly cross-linked 6% agarose

90-130 µmol Cl-/mL (media)

≥75mg BSA/mL (media)

E-CM-IE20

MMA Focurose 6HP

Highly cross-linked 6% agarose

130-200 µmol Cl-/mL (media)

≥95mg BSA/mL (media)

E-CM-IE22

MMC Focurose 6FF

Highly cross-linked 6% agarose

150-250 µmol H+/mL (media)

≥75 mg BSA/mL (media)

E-CM-IE23

MMC Focurose 6HP

Highly cross-linked 6% agarose

120-180 µmol H+/mL (media)

≥60 mg BSA/mL (media)

Hydrophobic interaction chromatography (HIC) is a useful method for purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied in the separation and purification of hydrophobic proteins such as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.

Cat. No.

Product name

Matrix

Ligand density

Binding capacity

E-CM-HI01

Phenyl Focurose 6FF (HS)

Highly cross-linked 6% agarose

40-50 µmol/mL

40 mg (IgG)/mL (media)

E-CM-HI02

Phenyl Focurose 6FF (LS)

Highly cross-linked 6% agarose

15-20 µmol/mL

15 mg (IgG)/mL (media)

E-CM-HI03

Phenyl Focurose 6HP

Highly cross-linked 6% agarose

25-30 µmol/mL

30 mg (IgG)/mL (media)

E-CM-HI04

Phenyl Focurose 6BB

Cross-linked 6% agarose

25-30 µmol/mL

25 mg (IgG)/mL (media)

E-CM-HI06

Butyl-S Focurose 6FF

Highly cross-linked 6% agarose

10 µmol/mL

10-20 mg (Lysyme)/mL (media)

E-CM-HI07

Octyl Focurose 4FF

Highly cross-linked 4% agarose

~5 µmol/mL

~10 mg (Lysyme)/mL (media)