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How to choose positive control for antibodies?

Source: Elabscience®Published: Jul 01,2024

The use of positive control is the basis for determining whether the antibody and detection system work normally in an experiment, and it is also a basis for determining whether the protein to be detected exists in the sample to be detected! Especially when the detected sample is uncertain whether the protein to be detected is expressed. Therefore, when there is no positive result in the experiment, it is best to choose the appropriate positive control, and most of the instructions have recommended positive controls; Or you can search according to the SwissProt or Omnigene database in the instructions, these databases often list the tissues in which the protein is highly expressed, and these samples can be used as suitable positive controls.