Antibody & Protein
Will the loading buffer affect the effectiveness of the electrophoresis solution? Do I need to replace the electrophoresis solution if I accidentally run the buffer out?
Source: Elabscience®Published: Aug 14,2024
The loading buffer will have an impact on the electrophoresis solution. If the overflow is small, it can be ignored. If there is a large overflow, it is necessary to replace the electrophoresis solution with fresh one in a timely manner to avoid affecting the subsequent electrophoresis effect.
To avoid buffer overflow, it is recommended to dissolve completely in a temperature bath below 37 ℃ before use.