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What is the reason why there is no precipitation when the protein added to the buffer solution is boiled, but there is precipitation when the sample is taken out and added the next day? How to solve it?

Source: Elabscience®Published: Aug 14,2024

The precipitation of denatured protein buffer may be related to the ion concentration in the protein sample. Precipitation occurs during temperature changes and can be dissolved by incubating in a water bath below 37 ℃. If there is still a small amount of sediment that cannot be dissolved, centrifuge and take the supernatant for use.