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Can DAPI be excited using laser confocal laser at λ=405nm after nuclear staining?

Source: Elabscience®Published: Aug 14,2024

DAPI can be excited using excitation light at 405 nanometers. Although the optimal excitation wavelength for DAPI is in the ultraviolet region (approximately 350-360 nanometers), it can also effectively excite under blue light at 405 nanometers. Using a 405 nanometer excitation light source can still activate DAPI and generate blue fluorescence for observing and imaging cell nuclei.