Antibody & Protein
Can DAPI be excited using laser confocal laser at λ=405nm after nuclear staining?
Source: Elabscience®Published: Aug 14,2024
DAPI can be excited using excitation light at 405 nanometers. Although the optimal excitation wavelength for DAPI is in the ultraviolet region (approximately 350-360 nanometers), it can also effectively excite under blue light at 405 nanometers. Using a 405 nanometer excitation light source can still activate DAPI and generate blue fluorescence for observing and imaging cell nuclei.