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After using 4% paraformaldehyde to fix cells at room temperature for 15 minutes, foaming occurred on the cell membrane. Do we need to change the fixed temperature to 4 ℃?

Source: Elabscience®Published: Aug 14,2024

The occurrence of foam on the cell membrane may be due to prolonged fixation time, and the fixation time can be appropriately shortened to optimize the fixation results.