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What is the concentration of absolute ethanol in the cell cycle kit? Can I also use 70%? Is it able to detect sub-G1 signal?

Source: Elabscience®Published: Mar 04,2024

Absolute ethanol is anhydrous ethanol which is 100%. It is not recommended to be replaced by 70% ethanol in order to match the best condition of assay. If there is a sub-G1 spike, it can be detected. Due to nucleus concentration and DNA fragmentation, some genomic DNA fragments of apoptotic cells were lost during the staining process. Therefore, apoptotic cells showed significantlly weaker staining after PI staining than that of the normal cells, that is, the fluorescence intensity was less than 1. The so-called sub-G1 peak, or apoptotic cell peak, appeared on the flow detection result map.