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Should it use serum medium or serum-free medium when opetaing cell cycle assays?

Source: Elabscience®Published: Mar 04,2024

Serum-free medium is necessary for cell cycle assays. Before the start of the experiment, cells should be cultured in serum-free medium for 12h to synchronize the cycle (pay attention to cell status, do not culture cells without serum for too long, or the cell state may be too poor to proliferate, end up with shrunk G2 phase or even sub-G1 apoptotic peak). Then normal medium was used for drug treatment, intervention and other experiments, and finally samples were collected for cell cycle detection.