Cell Metabolism
Preparation of Tissue Samples for Metabolism Assays
Source: Elabscience®Published: Jun 24,2021
This is a video about Preparation of Animal Tissue Samples for Metabolism Assays.
Take 0.02-1g fresh tissue to wash with homogenization medium at 2-8℃ to remove blood cells. Absorb the water with filter paper and weigh. Homogenize at the ratio of the volume of homogenized medium (2-8℃) (mL): the weight of the tissue (g) =9:1, then centrifuge the tissue homogenate for 10 min at 10000 g at 4℃. Take the supernatant to preserve it on ice for detection.
