ELISA Research
I saw that when the concentration of cAMP was measured by competitive ELISA kit in the literature, 0.1M HCl-lysed cells were used. Can we use it?
Source: Elabscience®Published: Mar 08,2024
We recommend that you refer to the cell sample treatment in the instructions instead of using 0.1M HCl for cell lysis.
In theory, 0.1M hydrochloric acid can only change the permeability of cells and cannot lyse cells. At the same time, the introduction of strong acids will lead to the degeneration of proteins, which will affect the binding of antigens and antibodies, and change the pH value of the ELISA experimental system, and ultimately affect the experimental results.