ELISA Research
How to perform ultrasonic processing?
Source: Elabscience®Published: Mar 05,2024
Using ultrasonic cell crusher, ultrasonic treatment of the suspension to lyse the cells (reference: Soniprep150 ultrasonic generator, ultrasonic treatment of 30s at an amplitude of 14μm, cell fragmentation; Or use an ultrasonic crusher, 200W, 2s/ times, gap 3s, total time 3-5min or 400 amp, 5s/ times, gap 10s, repeated 3-5 times), and then centrifuge at 2 to 8℃ at 1500×g for 10min, remove the cell debris, collect the supernatant. Attention should be paid to temperature during ultrasound.