Flow Cytometry
How should experimental groups/controls be set?
Source: Elabscience®Published: Mar 04,2024
Blank control: used to set the voltage of each channel.
Isotype control: Isotype control antibodies are used as the basis for determining negative and positive cells. It is necessart as a gating helper especially for the indicators with low expression or continuous expression.
Single color control: In a multicolor experiment, single color control is needed to adjust the fluorescence compensation if there is interference between the different channels.
FMO control: FMO control, also known as fluorescence reduction control, refers to the multi-color experiment. FMO control is applied to observe the comprehensive effect of all related fluoresents to the target channel by removing the correspinding signal.