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How should experimental groups/controls be set?

Source: Elabscience®Published: Mar 04,2024

Blank control: used to set the voltage of each channel. Isotype control: Isotype control antibodies are used as the basis for determining negative and positive cells. It is necessart as a gating helper especially for the indicators with low expression or continuous expression. Single color control: In a multicolor experiment, single color control is needed to adjust the fluorescence compensation if there is interference between the different channels. FMO control: FMO control, also known as fluorescence reduction control, refers to the multi-color experiment. FMO control is applied to observe the comprehensive effect of all related fluoresents to the target channel by removing the correspinding signal.