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OT(Oxytocin) ELISA Kit

Cat.No.:E-EL-0029
Reactivity: Universal

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes OT in samples. No significant cross-reactivity or interference between OT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of OT concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OT. During the reaction, OT in the sample or standard competes with a fixed amount of OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OT in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level OT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level OT were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	53.20	153.88	495.39	56.27	139.06	544.58
Standard deviation	2.78	7.02	16.10	2.97	6.04	23.85
CV (%)	5.23	4.56	3.25	5.28	4.34	4.38

Recovery

The recovery of OT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-103	98
EDTA plasma(n=8)	92-104	99
Cell culture media(n=8)	88-102	94

Linearity

Samples were spiked with high concentrations of OT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Neuroscience, Biochemicals, Signal Transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

ACS Applied Materials & Interfaces (2022) IF: 9.2289
Oxytocin Nanogels Inhibit Innate Inflammatory Response for Early Intervention in Alzheimer’s Disease

DOI: 10.1021/acsami.2c00007

PMID: 35510352
Sample: brain
Frontiers in Psychiatry (2022) IF: 5.435
Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.3389/fpsyt.2022.919284

PMID: 36032243
Sample: serum
Scientific Reports (2019) IF: 4.011
Protective effect of oxytocin on LPS-induced acute lung injury in mice

DOI: 10.1038/s41598-019-39349-1

PMID: 30808956
Sample: Tissue homogenate
JOURNAL OF PSYCHOPHARMACOLOGY (2019) IF: 4.221
Social isolation rearing-induced anxiety and response to agomelatine in male and female rats: Role of corticosterone, oxytocin, and vasopressin:

DOI: 10.1177/0269881119826783

Sample: Plasma
ACS Chemical Neuroscience (2022) IF: 4.418
Protective Effects of Intranasally Administrated Oxytocin-Loaded Nanoparticles on Pentylenetetrazole-Kindling Epilepsy in Terms of Seizure Severity, Memory, Neurogenesis, and Neuronal Damage

DOI: 10.1021/acschemneuro.2c00124

PMID: 35713692
Sample: serum
BEHAVIOURAL BRAIN RESEARCH (2018) IF: 3.173
The effects of acute foot shock stress on empathy levels in rats

DOI: 10.1016/j.bbr.2018.04.043

PMID: 29709611
Sample: Serum,Tissue homogenate
BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

DOI: 10.1016/j.bbr.2022.114082

PMID: 36041571
Sample: serum
Neuropsychiatric Disease and Treatment (2018) IF: 2.195
Atrial natriuretic peptide and posterior pituitary neurohormone changes in patients with acute schizophrenia

DOI: 10.2147/NDT.S169619

Sample: Serum
BIOLOGICAL & PHARMACEUTICAL BULLETIN (2021) IF: 2.233
Targeting the oxytocin system to ameliorate early life depressive-like behaviors in maternally-separated rats

DOI: 10.1248/bpb.b21-00247

PMID: 34349049
Sample: plasma
Psychiatry Investigation (2022) IF: 2.505
The Relationship Between the Oxytocin Level and Rejection Sensitivity, Childhood Traumas, and Attachment Styles in Borderline Personality Disorder

DOI: 10.30773/pi.2021.0358

PMID: 35330564
Sample: Serum
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2018) IF: 2.538
Acetaminophen (paracetamol) affects empathy-like behavior in rats: Dose-response relationship

DOI: 10.1016/j.pbb.2018.10.004

PMID: 30359628
Sample: Serum,Tissue homogenate
Balkan Medical Journal (2019) IF: 1.203
Regular Aerobic Voluntary Exercise Increased Oxytocin in Female Mice: The Cause of Decreased Anxiety and Increased Empathy-Like Behaviors

DOI: 10.4274/balkanmedj.galenos.2019.2018.12.87

Sample: blood,brain
Journal of Maternal-Fetal & Neonatal Medicine (2015) IF: 1.367
Immediate breastfeeding and skin-to-skin contact during cesarean section decreases maternal oxidative stress, a prospective randomized case-controlled study

DOI: 10.3109/14767058.2015.1101447

Sample: Serum
ANIMAL REPRODUCTION SCIENCE (2020) IF: 1.66
Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance

DOI: 10.1016/j.anireprosci.2020.106531

PMID: 32828406
Sample: Serum
Veterinary World (2020)
Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa

DOI: 10.14202/vetworld.2020.502-507

PMID: 32367956
Sample: Serum
Journal of General and Family Medicine (2023)
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial

DOI: 10.1002/jgf2.606

Sample: serum
IBRO Neuroscience Reports (2022)
The Effect of Oxytocin and an Enriched Environment on Anxiety-Like Behaviour and Corticosterone Levels in a Prenatally Stressed Febrile Seizure Rat Model

DOI: 10.1016/j.ibneur.2022.05.001

Sample: Hypothalamic tissue
IBRO Neuroscience Reports (2021)
Prolonged efavirenz exposure reduces peripheral oxytocin and vasopressin comparable to known drugs of addiction in male Sprague Dawley rats

DOI: 10.1016/j.ibneur.2021.06.003

Sample: plasma
Sleep and Biological Rhythms (2018) IF: 0.655
Evaluation of behavior and endocrinological changes after REM sleep deprivation-induced mania-like behavior in mice

DOI: 10.1007/s41105-018-0166-6

Sample: serum

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Reviews/Q&A

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Reviews
Q&A

G*********gSubmitted [ Mar 14 2023 ]

Asked: Does this assay measure oxytocin accurately in unextracted plasma samples - i.e. is there any plasma matrix interference?

adminSubmitted [ Mar 14 2023 ]

Answered: This kit can detect anticoagulant plasma samples, rather than unextracted plasma samples, and EDTA-2Na is generally recommended as an anticoagulant. There are many factors causing matrix interference, such as repeated freeze-thaw or hemolysis samples, which will lead to inaccurate test results. Therefore, in order to ensure the accuracy of the results, it is recommended to use fresh samples as much as possible and avoid using repeated freeze-thaw and hemolysis plasma samples.

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