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Human SOST(Sclerostin) ELISA Kit

Citations(9)Uniprot : Q9BQB4

Cat.No.:E-EL-H1544
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human SOST in samples. No significant cross-reactivity or interference between Human SOST and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human SOST concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOST. You can calculate the concentration of Human SOST in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SOST were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SOST were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	222.11	370.69	1524.64	203.00	389.31	1557.60
Standard deviation	11.82	15.05	67.24	11.27	21.30	67.60
CV (%)	5.32	4.06	4.41	5.55	5.47	4.34

Recovery

The recovery of Human SOST spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-99	91
EDTA plasma (n=8)	92-107	98
Cell culture media (n=8)	88-103	94

Linearity

Samples were spiked with high concentrations of Human SOST and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9BQB4
Synonyms	CDD, VBCH
Research Area	Cancer, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF PERIODONTOLOGY (2021) IF: 6.993
Evaluation of GCF IL-17, IL-10, TWEAK and sclerostin levels after SRP and adjunctive use of diode laser application in periodontitis patients

DOI: 10.1002/JPER.21-0494

Sample: gingival crevicular fluid (GCF)
CYTOKINE (2018) IF: 3.514
Evaluation of gingival crevicular fluid and peri-implant crevicular fluid levels of sclerostin, TWEAK, RANKL and OPG

DOI: 10.1016/j.cyto.2018.10.021

PMID: 30377055
Sample: Gingival Crevicular Fluid
RHEUMATOLOGY INTERNATIONAL (2019) IF: 2.2
Evaluation of serum fibroblast growth factor-23 in patients with axial spondyloarthritis and its association with sclerostin, inflammation, and spinal damage

DOI: 10.1007/s00296-019-04298-5

PMID: 30968207
Sample: Serum
Medicina-Lithuania (2020) IF: 1.205
Significance of acPWV for Survival of Hemodialysis Patients

DOI: 10.3390/medicina56090435

PMID: 32872092
Sample: Serum
JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM (2020) IF: 1.278
Sclerostin and osteoprotegerin: new markers of chronic kidney disease mediated mineral and bone disease in children

DOI: 10.1515/jpem-2020-0140

Sample: serum
Journal of Maternal-Fetal & Neonatal Medicine (2018) IF: 1.493
Inhibitors of osteoblastogenesis in early human milk and maternal serum: evidence for protective properties of mother’s milk on bone

DOI: 10.1080/14767058.2018.1514383

Sample: Serum,Milk
NEFROLOGIA (2020) IF: 1.544
The correlation between sclerostin and bone mineral density in renal transplant recipients

DOI: 10.1016/j.nefro.2020.04.009

PMID: 32896441
Sample: Serum
INTERNATIONAL UROLOGY AND NEPHROLOGY (2018) IF: 1.692
Is serum sclerostin a marker of atherosclerosis in patients with chronic kidney disease–mineral and bone disorder?

DOI: 10.1007/s11255-018-1935-5

Sample: Serum
Contemporary Clinical Dentistry (2021)
Comparative Evaluation of Sclerostin Levels in Gingival Crevicular Fluid in the Treatment of Chronic Periodontitis Patients Using Diode Laser as an Adjunct to Scaling and Root Planing: A Clinico-biochemical Study

DOI: 10.4103/ccd.ccd_19_20

Sample: gingival crevicular fluid (GCF)

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