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Cat.No.:E-EL-H0910
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Format | 24T, 48T, 96T, 96T*5, 96T*10 |
Assay time | 3.5h |
Sample type | serum, plasma and other biological fluids |
Sample volume | 100μL |
Sample size | Run up to 46 samples in duplicate and 30 samples in triplicate |
Storage | 2-8℃ |
Interpretation | Detection range: 78.13-5000 pg/mL Sensitivity: 46.88 pg/mL |
Application | Quantitative determination of Human concentrations in human serum, plasma and other biological fluids |
Reproducibility | Both intra-CV and inter-CV are < 10% |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Sample: Serum |
DOI: 10.4314/ahs.v22i1.39 |
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PMID: 36032449 |
Sample: Plasma |
Sample: Cell Lysate |
PMID: 32138726 |
Sample: Saliva |
PMID: 30098371 |
Sample: Whole Blood |
DOI: 10.3390/ijms23095019 |
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PMID: 35563410 |
Sample: Pbmcs |
PMID: 35164509 |
Sample: Mcf-7 Cell |
T***oSubmitted [ May 14 2024 ]
Asked: Is this catalog no: E-EL-H0910 for wild type p53 or mutant p53?
Reply
adminSubmitted [ May 14 2024 ]
Answered: Hi, E-EL-H0910 si for wild type p53, thank you!