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Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Citations(10)Uniprot : P35354

Cat.No.:E-EL-H1846
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Human PTGS2/COX-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTGS2/COX-2. You can calculate the concentration of Human PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PTGS2/COX-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PTGS2/COX-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.98	1.83	7.54	0.93	2.01	7.68
Standard deviation	0.06	0.09	0.32	0.06	0.08	0.38
CV (%)	6.12	4.92	4.24	6.45	3.98	4.95

Recovery

The recovery of Human PTGS2/COX-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-102	94
EDTA plasma (n=8)	95-108	100
Cell culture media (n=8)	86-101	92

Linearity

Samples were spiked with high concentrations of Human PTGS2/COX-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P35354
Synonyms	COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nutrients (2021) IF: 5.719
Effects of 12 Weeks Cosmos caudatus Supplement among Older Adults with Mild Cognitive Impairment: A Randomized, Double-Blind and Placebo-Controlled Trial

DOI: 10.3390/nu13020434

PMID: 33572715
Sample: Serum
Frontiers in Pharmacology (2022) IF: 5.988
Metabolomics Profiles Associated with the Treatment of Zuojin Pill on Patients with Chronic Nonatrophic Gastritis

DOI: 10.3389/fphar.2022.898680

PMID: 35899115
Sample: serum
Biomolecules (2020) IF: 4.082
Benzimidazole Containing Acetamide Derivatives Attenuate Neuroinflammation and Oxidative Stress in Ethanol-Induced Neurodegeneration

DOI: 10.3390/biom10010108

PMID: 31936383
Sample: Tissue homogenate
Frontiers in Pharmacology (2020) IF: 4.225
GEO Data Sets Analysis Identifies COX-2 and Its Related Micro RNAs as Biomarkers for Non-Ischemic Heart Failure

DOI: 10.3389/fphar.2020.01155

PMID: 32848764
Sample: Plasma
Aging-US (2020) IF: 4.831
Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure

DOI: 10.18632/aging.103827

PMID: 33159018
Sample: Serum
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
Potential Therapeutic Activity of Berberine in Thyroid-Associated Ophthalmopathy: Inhibitory Effects on Tissue Remodeling in Orbital Fibroblasts

DOI: 10.1167/iovs.63.10.6

PMID: 36094643
Sample: Orbital fibroblasts (OFs)
MOLECULES (2022) IF: 4.927
Modulatory Effect of Diosmin and Diosmetin on Metalloproteinase Activity and Inflammatory Mediators in Human Skin Fibroblasts Treated with Lipopolysaccharide

DOI: 10.3390/molecules27134264

PMID: 35807509
Sample: human fibroblast cell
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS (2021) IF: 2.512
Potential associations between variants of genes encoding regulators of inflammation, and mediators of inflammation in type 2 diabetes and insulin resistance

DOI: 10.1111/jcpt.13471

PMID: 34145611
Sample: serum
BMC Complementary and Alternative Medicine (2020) IF: 2.833
The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial

DOI: 10.1186/s12906-020-03092-2

PMID: 33076878
Sample: Serum
PROSTAGLANDINS & OTHER LIPID MEDIATORS (2016) IF: 2.905
Decreased eicosapentaenoic acid levels in acne vulgaris reveals the presence of a proinflammatory state

DOI: 10.1016/j.prostaglandins.2016.12.001

Sample: Serum

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