Current Position:Home>>Kits>>ELISA Kits>> 4-HNE(4-Hydroxynonenal) ELISA Kit
Cat.No.:E-EL-0128
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 2.0h |
Detection range | 0.63-40 ng/mL |
Sensitivity | 0.38 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of 4-HNE concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 4-HNE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 4-HNE were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 1.90 | 5.40 | 18.00 | 1.90 | 5.80 | 19.10 |
Standard deviation | 0.10 | 0.20 | 0.60 | 0.10 | 0.30 | 0.60 |
CV (%) | 5.26 | 3.70 | 3.33 | 5.26 | 5.17 | 3.14 |
The recovery of 4-HNE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 89-102 | 94 |
EDTA plasma(n=8) | 91-107 | 99 |
Cell culture media(n=8) | 92-106 | 100 |
Samples were spiked with high concentrations of 4-HNE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Research Area | Neuroscience, Cell Biology, Metabolism |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
Sample: Thp-1 Cell |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
PMID: 31850805 |
Sample: Tissue Homogenate |
Sample: Plasma,Serum |
PMID: 30890312 |
Sample: Tissue Homogenate |
PMID: 33610458 |
Sample: Serum,Tissue Homogenate(Testis) |
PMID: 32705206 |
Sample: Serum |
PMID: 30217444 |
Sample: Cell Culture Supernatant |
DOI: 10.1155/2018/4135104 |
|
Sample: Plasma |
PMID: 33710598 |
Sample: Cell Sample |
DOI: 10.1017/neu.2020.19 |
|
Sample: Hippocampal Tissue |
DOI: 10.1155/2021/6696806 |
|
PMID: 34257742 |
Sample: Plasma |
PMID: 30797748 |
Sample: Serum |
Sample: Liver |
DOI: 10.3390/nu11020228 |
|
PMID: 30678193 |
Sample: Plasma |
DOI: 10.3390/biom12010048 |
|
PMID: 35053196 |
Sample: Ce81T Cell,Te1 Cell |
DOI: 10.1155/2020/5849794 |
|
Sample: Tissue Homogenate |
Sample: Serum,Liver Tissue,Aml-12 Cell |
Sample: Cell Culture Supernatant,Cell Lysate |
Sample: Bend.3 Cell |
PMID: 33805825 |
Sample: Serum |
Sample: Hacat Cell |
DOI: 10.1155/2023/8258354 |
|
Sample: Jejunal Tissue |
PMID: 35038550 |
Sample: A549 Cell |
PMID: 36122518 |
Sample: Gastric Mucosal Tissue, |
Sample: Liver |
PMID: 36087883 |
Sample: Hippocampus Tissue,Ht22 Cell. |
PMID: 35422292 |
Sample: Kidney |
PMID: 34517184 |
Sample: H1975 Cell |
PMID: 35961822 |
Sample: Primary Kupffer Cell |
Sample: 4T1 Cell |
M*MSubmitted [ Jun 15 2021 ]
Asked: Do you know if it works with tissue samples?
Reply
adminSubmitted [ Jun 15 2021 ]
Answered: This kit can be used for tissue samples.