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Cat.No.:E-AB-F1140E
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Alternate Names | CD27L receptor,T-cell activation antigen CD27,T14,S152,TNFRSF7 |
Swissprot | P26842 |
References |
1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627. |
Form | Liquid |
Clone No. | O323(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | APC |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5.5 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® Red 780 Anti-Human CD27 Antibody[O323] | 20Tests, 100Tests, 100Tests×2 | Check More |