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Cat.No.:E-BC-K236-M
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Synonyms | AST,GOT,Glutamic-oxalacetic transaminase |
Sample type | Serum,plasma,animal tissue,cells,cell culture supernatant |
Sensitivity | 1.1 IU/L |
Detection range | 1.1-72.3 IU/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 80 min |
Precision | Average inter-assay CV: 6.800%Average intra-assay CV: 5.300% |
Other instruments required | Micropipettor, Vortex mixer, Incubator, Multichannel pipette |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
No significant difference of AST activity was observed between WT and 3SA mice after HFD for 17 weeks.
H Xia et al investigate the relationship between insulin resistance and GSK3β-FBXW7-ERRα transcriptional axis. Aspartate aminotransferase (AST) activity in mouse serum was determined using AST activity assay kit (E-BC-K236-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (1.1-72.3 IU/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Porcine serum |
1 |
Rat serum |
1 |
HC-60 cellular supernatant |
1 |
Calu-3 cellular supernatant |
1 |
10% Rat liver tissue homogenization |
15-30 |
10% Rat lung tissue homogenization |
2-8 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
PMID: 36808719 |
Sample: Serum |
DOI: 10.1002/hep.29573 |
|
Sample: serum |
PMID: 35440636 |
Sample: serum |
PMID: 36090760 |
Sample: plasma |
Sample: plasma |
PMID: 33360192 |
PMID: 34448397 |
Sample: plasma |
PMID: 36103835 |
Sample: serum |
Sample: blood |
DOI: 10.1111/cpr.13332 |
|
Sample: serum |
PMID: 36058506 |
Sample: serum |
PMID: 34757230 |
Sample: serum |
Sample: Serum,Plasma |
Sample: serum |
PMID: 34571217 |
Sample: liver organoids (LOs) |
PMID: 35588832 |
Sample: serum |
PMID: 33727180 |
Sample: Serum |
PMID: 36293338 |
Sample: blood |
PMID: 36068769 |
Sample: Serum |
Sample: Serum |
PMID: 35341826 |
Sample: serum |
Sample: Serum |
Sample: serum |
PMID: 36230124 |
Sample: serum |
Sample: serum |
PMID: 34483906 |
Sample: liver |
DOI: 10.3390/ijms23105490 |
|
Sample: serum |
PMID: 34681767 |
PMID: 35035768 |
Sample: Blood |
Sample: blood |
PMID: 35443217 |
Sample: serum |
DOI: 10.1039/D1DT00185J |
|
PMID: 33982702 |
Sample: Serum |
DOI: 10.1111/jcmm.15614 |
|
PMID: 32678475 |
Sample: Serum |
PMID: 33827384 |
Sample: serum |
Sample: serum |
PMID: 34717194 |
Sample: serum |
Sample: serum |
DOI: 10.1155/2022/9615674 |
|
PMID: 35692375 |
Sample: plasma |
PMID: 31824338 |
Sample: Plasma |
PMID: 35010758 |
Sample: plasma |
DOI: 10.1002/jbt.23177 |
|
PMID: 35983967 |
Sample: serum |
PMID: 35298730 |
Sample: serum |
PMID: 35277165 |
Sample: serum |
PMID: 32705206 |
Sample: Serum |
DOI: 10.1155/2022/7832540 |
|
Sample: hepatic tissue,serum |
Sample: Serum |
DOI: 10.7717/peerj.13082 |
|
PMID: 35310165 |
Sample: plasma |
Sample: serum |
DOI: 10.5650/jos.ess22033 |
|
PMID: 35965085 |
Sample: liver |
Sample: plasma |
Sample: serum |
Sample: Serum |
Sample: plasma |
Sample: plasma |
Verified Customer
a*****hSubmitted [ Jun 05 2019 ]
M*****aSubmitted [ Oct 20 2022 ]
Asked: Is it possible to perform this AST activity assay on frozen samples. For example if I have done the sample preparation on mouse livers, if I store the extracted samples at -80°C for a few nights can I reuse those same samples for the enzyme activity test?
Reply
adminSubmitted [ Oct 20 2022 ]
Answered: If you has no time to test on the same day, the tissue can be aliquoted and frozen at -80 degrees. If the supernatant after homogenization is processed, it is not recommended to test overnight, which will affect the enzyme activity. The tissue homogenization can be performed when you needs to test.
M*****aSubmitted [ Sep 20 2022 ]
Asked: I want to know what is the exact difference between the sample and the control reactions. Both wells have all the same reagents added to them and also both have the sample. The sample is just added at different times, does this cause a change in the reaction?
Reply
adminSubmitted [ Sep 20 2022 ]
Answered: Set the control is to exclude the interference of pyruvate and non-enzymatic reaction of the sample. Reagent 4 has an inhibitory effect on the enzyme and can terminate the reaction between substrate and enzyme.
s************fSubmitted [ Apr 01 2022 ]
Asked: What is meant by 98T or 48T
Reply
adminSubmitted [ Apr 01 2022 ]
Answered: Hi sumaira, Thank you for your effort, we will reply to your email soon.