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Cat.No.:E-BC-K103-M
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Protocols for metabolism assays
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Synonyms | Ca |
Sample type | Serum,plasma,urine,tissue |
Sensitivity | 0.07 mmol/L |
Detection range | 0.07-1.2 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 8.500%Average intra-assay CV: 4.700% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Storage | 2-8℃ |
Valid period | 12 months |
The levels of Ca concentration was significantly reduced in PD5 group. (**P<0.01)
TH Huang et al developed a surface decorated peptide nanoparticle that can be used for osteoporosis and diabetes therapy. Calcium (Ca) level in rat plasma was determined using Ca colorimetric assay kit (E-BC-K103-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-1.2 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Dog serum |
2-3 |
Human serum |
3-6 |
Mouse serum |
3-6 |
Human urine |
4-8 |
20% Animal tissue homogenate |
1 |
Note: The diluent is deionized water.
Sample: plasma |
PMID: 36283558 |
Sample: STC1 cells |
PMID: 36209841 |
Sample: serum |
PMID: 36049670 |
Sample: serum |
PMID: 34478973 |
Sample: lung |
PMID: 35909740 |
Sample: roots or shoots |
Sample: brain |
Sample: colon |
Sample: Serum |
PMID: 32528892 |
Sample: Cell lysate |
PMID: 32339907 |
Sample: Serum |
PMID: 33130047 |
Sample: Tissue homogenate |
DOI: 10.3390/ani11030886 |
|
PMID: 33804672 |
Sample: Serum |
R******PSubmitted [ Dec 12 2023 ]
Asked: What is the dilution factor for Cell culture supernatants?
Reply
adminSubmitted [ Dec 12 2023 ]
Answered: Hello Ramesh,our laboratory verified the Ca concentration in HepG2 cell supernatant, diluted by 2 times and measured to 1.340 mmol/L. However, there are differences between different samples, it is recommended that you choose 2-3 samples for pre-experiment to determine the dilution factor.
M***********gSubmitted [ Jul 24 2023 ]
Asked: May I ask if I can analyse calcium content using cell lysate samples? I didn\'t see this sample being included in the protocol so I would like to ask further.
Reply
adminSubmitted [ Jul 24 2023 ]
Answered: Hi Sir, I'll reply to your mail
M*****iSubmitted [ Oct 27 2022 ]
Asked: What anticoagulant should be used for plasma sample?
Reply
adminSubmitted [ Oct 27 2022 ]
Answered: It is recommended to use heparin as an anticoagulant, do not use calcium chelating agent and oxalate.
M*****iSubmitted [ Sep 20 2022 ]
Asked: The reagent 3 is kind of solid. What should I do to use it?
Reply
adminSubmitted [ Sep 20 2022 ]
Answered: Preheat the reagent 3 at 37℃ until clarified before use.