Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.

General Information

Application	Caspase
Assay type	Qualitative or quantitative
Detection method	Colorimetric Method
Sample type	Cell and tissue samples
Assay time	4-6 hours
Detection instrument	Spectrophotometer
Other reagents required	PBS(E-BC-R187)，Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M)
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A382	Caspase 2 Activity Assay Kit(Colorimetric Method)
E-CK-A384	Caspase 4 Activity Assay Kit(Colorimetric Method)
E-CK-A386	Caspase 6 Activity Assay Kit(Colorimetric Method)

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