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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

  • Cat.No.:E-BC-K790-M

  • Detection instrument: Microplate reader (430-470 nm, optimum wavelength: 450 nm)

To Purchase E-BC-K790-M

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  • 96T
  • 48T
Price: $580
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Detection principle

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.

Performance characteristics

Synonyms GAPDH
Sample type tissue;cell
Sensitivity 0.49 U/L
Detection range 0.49-50.00 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 50 min
Precision Average inter-assay CV: 3.2-8.6%Average intra-assay CV: 3.2-5%
Other instruments required Incubator (37℃)
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months

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