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Hydroxyproline (HYP) Colorimetric Assay Kit (Alkali hydrolysis Method)

Cat.No.:E-BC-K061-S
Detection instrument: Spectrophotometer(550 nm)

Detection method: Colorimetric method

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Detection principle

The oxidation product which produced by hydroxyproline under the action of oxidant react with dimethylaminobenzaldehyde and show a purplish red color. The content of hydroxyproline can be calculated by measuring the OD value at 550 nm.

Performance characteristics

Synonyms	Hyp
Sample type	Serum (plasma),tissue,culture supernatant,body fluids
Sensitivity	0.01μg/mL
Detection range	0.01-20μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF CONTROLLED RELEASE (2017) IF: 7.786

Hepatic stellate cell-targeted imatinib nanomedicine versus conventional imatinib: A novel strategy with potent efficacy in experimental liver fibrosis

DOI: 10.1016/j.jconrel.2017.09.035
	Sample: liver tissue

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE (2019) IF: 2.467

A novel bilayer zein/MMT nanocomposite incorporated with H. perforatum oil for wound healing

DOI: 10.1007/s10856-019-6332-9
PMID: 31838599	Sample: Plant Fluid

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63

Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis

DOI: 10.1155/2022/4194827
PMID: 35774743	Sample: kidney

Gastroenterology Research and Practice (2020) IF: 1.806

Comparison of Platelet-Rich Plasma-Impregnated Suture Material with Low and High Platelet Concentration to Improve Colonic Anastomotic Wound Healing in Rats

DOI: 10.1155/2020/7386285
PMID: 32565785	Sample: Tissue homogenate

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Reviews/Q&A

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Q&A

L**********aSubmitted [ Apr 10 2024 ]

Asked: Ola! Gostaria de reber o método para uso do kit de detecção

adminSubmitted [ Apr 10 2024 ]

Answered: Thanks for your message. We will send the instructions to you and please check your email box.

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