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Cat.No.:E-EL-M0051
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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | Serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse TGF-β1 in samples. No significant cross-reactivity or interference between Mouse TGF-β1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse TGF-β1 concentrations in Serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TGF-β1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TGF-β1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 95.20 | 254.00 | 667.10 | 95.80 | 270.00 | 634.70 |
Standard deviation | 5.10 | 11.40 | 28.70 | 5.70 | 11.10 | 31.10 |
CV (%) | 5.36 | 4.49 | 4.30 | 5.95 | 4.11 | 4.90 |
The recovery of Mouse TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 93-109 | 101 |
EDTA plasma (n=8) | 93-110 | 101 |
Cell culture media (n=8) | 93-107 | 99 |
Synonyms | TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1 |
Research Area | Cancer,Cardiovascular,Metabolism,Neuroscience,Stem cells |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Sample: Serum,Raw264.7 Cell |
Sample: 4T1 Cell-Xenograft |
PMID: 36091371 |
Sample: Lung,Serum,Testis |
Sample: Skin |
Sample: Calvariae Origin Osteoblastic Cell Line Subclone 4 |
Sample: Cell Culture Supernatant |
PMID: 32741881 |
Sample: Serum |
PMID: 32442663 |
Sample: Serum |
DOI: 10.7717/peerj.13257 |
|
PMID: 35673387 |
Sample: Sv40 Mes 13 Cell |
Sample: Cell Culture Medium |
Sample: Blood |
PMID: 32774762 |
Sample: Serum,Balf |
Sample: Raw264.7 Cells |
DOI: 10.2147/DDDT.S351092 |
|
PMID: 35698653 |
Sample: Liver |
DOI: 10.3390/nu11020395 |
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PMID: 30781895 |
Sample: Tissue Homogenate |
PMID: 36253797 |
Sample: Serum |
Sample: Serum |
DOI: 10.1111/jcmm.15614 |
|
PMID: 32678475 |
Sample: Serum |
DOI: 10.1111/jcmm.15434 |
|
Sample: Plasma |
Sample: Serum,Tissue Homogenate |
PMID: 35149293 |
Sample: Macrophage |
Sample: Serum |
DOI: 10.1155/2022/5522828 |
|
Sample: Cd4+ T Cell |
PMID: 35777266 |
Sample: Colon Tissue |
PMID: 32114243 |
Sample: Tissue Homogenate |
DOI: 10.1155/2022/5249367 |
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PMID: 35422896 |
Sample: Plasma |
Sample: Interstitial Fluid |
Sample: Spleen Cells |
DOI: 10.1039/D2TB00527A |
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Sample: Raw 264.7 Cell |
DOI: 10.7150/thno.31461 |
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Sample: Mammary Gland Tumor Tissue,Plasma |
DOI: 10.1111/cpr.13316 |
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PMID: 35869570 |
Sample: Skin Samples |
PMID: 35860242 |
Sample: Serum,Prostate Tissue |
Sample: Hippocampal Tissue |
PMID: 34119557 |
Sample: Lung |
PMID: 36314479 |
Sample: Hepa 1-6 Cells-Xenograft |