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Cat.No.:E-BC-K035-S
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Synonyms | NO |
Sample type | Serum,plasma,tissue |
Sensitivity | 0.97 μmol/L |
Detection range | 0.97-700 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 80 min |
Precision | Average inter-assay CV: 5.200%Average intra-assay CV: 3.400% |
Other instruments required | Vortex mixer, Centrifuge, Analytical Balance, Micropipettor |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 6: -20℃, others: 2-8℃ |
Valid period | 12 months |
NO level was significantly reduced by cold exposure. (P<0.01)
BYWng et al investigate the mechanism between cold exposure, gut microbiota and hypertension. Nitric oxide (NO) level of rat plasma was determined using NO colorimetric assay kit (E-BC-K035-S).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-700 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
10% Mouse liver tissue homogenization |
1 |
Rat serum |
1 |
Rat plasma |
1 |
10% Epipremnum aureum tissue homogenization |
1 |
Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
PMID: 35697111 |
Sample: embryo |
Sample: neutrophils |
PMID: 34517076 |
Sample: astrocyte |
PMID: 35277106 |
Sample: RAW 264.7 cell |
PMID: 34399203 |
Sample: heart |
Sample: cardiac microvascular endothelial cells (CMECs) |
PMID: 34197855 |
Sample: RAW264.7 cells |
PMID: 34054541 |
Sample: Cell culture supernatant |
Sample: RAW264.7 |
PMID: 36157749 |
Sample: serum |
PMID: 35280546 |
Sample: MCF-7 cell |
Sample: colon,liver |
Sample: Serum |
PMID: 35306040 |
Sample: liver homogenates |
DOI: 10.1111/andr.13218 |
|
PMID: 35765205 |
Sample: penile cavernous tissue |
PMID: 32570115 |
Sample: Cell sample |
Sample: hippocampa |
Sample: peritoneal macrophages (PMs) |
DOI: 10.3390/ani12233245 |
|
Sample: skimmed milk |
DOI: 10.3390/ani12162125 |
|
PMID: 36009715 |
Sample: serum |
DOI: 10.1111/andr.13188 |
|
PMID: 35466583 |
Sample: penile cavernous tissue |
PMID: 33300058 |
Sample: Cell culture supernatant |
Sample: HUVEC |
PMID: 32550799 |
Sample: Serum |
PMID: 33211294 |
Sample: lvier |
Sample: PMs cells |
Sample: macrophages |
Sample: serum,sciatic nerves |
Sample: Serum |
J****eSubmitted [ Mar 10 2023 ]
Asked: Can this kit use for NO measurement in oil (not water solvent)?
Reply
adminSubmitted [ Mar 10 2023 ]
Answered: Sorry, this kit can not use for oil.
M*****iSubmitted [ Sep 08 2022 ]
Asked: How long can the chromogenic reagent be stored after it is prepared?
Reply
adminSubmitted [ Sep 08 2022 ]
Answered: It is recommended to use it within half a day