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Cat.No.:E-BC-K117-S
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Sample type | Serum,plasma,hydrothorax,cell culture supernatant,tissue |
Sensitivity | 0.02 nmol/mgprot |
Detection range | 0.02-10 nmol/mgprot |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 240 min |
Precision | Average inter-assay CV: 8.600%Average intra-assay CV: 4.500% |
Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Other reagents required | Absolute ethanol, Ethyl acetate |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment. The sample can be detected by this kit when the protein content of samples is ranged from 1-10 mg/mL.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
8-10 |
Mouse serum |
8-10 |
10% Rat liver tissue homogenization |
2-3 |
10% Mouse heart tissue homogenization |
1 |
10% Mouse brain tissue homogenization |
1 |
Note: The diluent is double distilled water or reagent 1.
PMID: 34768063 |
Sample: plasma |
Sample: plasma |
DOI: 10.3390/ph15010016 |
|
Sample: serum |
PMID: 31279280 |
Sample: Tissue homogenate |
Sample: liver |
PMID: 32220623 |
Sample: Tissue homogenate |
DOI: 10.1002/jez.2603 |
|
Sample: brain |
M**********dSubmitted [ Aug 18 2022 ]
Asked: Is it possible to measure virus solution?
Reply
adminSubmitted [ Aug 18 2022 ]
Answered:
D********dSubmitted [ Aug 02 2022 ]
Asked: Is the sample of cytoplasm avaliable?
Reply
adminSubmitted [ Aug 02 2022 ]
Answered: Unfortunately, the concentration of protein carbonyl in cytoplasm is too low to be measured with this kit.
a********** Submitted [ Mar 02 2019 ]
Asked: what is the normal rang value ? I got the value 0.353 . is this wrong ? and why ?
Reply