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Rat TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

Citations(40)

  • Cat.No.:E-EL-R0084

  • Reactivity: Rat
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  • 96T
  • 24T
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume Serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat TGF-β1 in samples. No significant cross-reactivity or interference between Rat TGF-β1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat TGF-β1 concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TGF-β1. You can calculate the concentration of Rat TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TGF-β1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TGF-β1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 104.20 170.70 940.70 112.60 182.50 874.90
Standard deviation 6.00 7.30 38.60 7.40 8.90 34.10
CV (%) 5.76 4.28 4.10 6.57 4.88 3.90

Recovery

The recovery of Rat TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 94
EDTA plasma (n=8) 86-101 93
Cell culture media (n=8) 93-107 99

Target Information

SynonymsTGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1
Research AreaCancer,Cardiovascular,Metabolism,Neuroscience,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Advanced Healthcare Materials (2022) IF: 11.092
    Spatiotemporal regulation of the bone immune microenvironment via dam-like biphasic bionic periosteum for bone regeneration

    DOI: 10.1002/adhm.202201661

    PMID: 36189833

    		 Sample: BMSCs
  2. BRITISH JOURNAL OF PHARMACOLOGY (2022) IF: 8.74
    Resveratrol attenuates behavioural impairment associated with learning and memory in HFD-STZ induced diabetic rats

    DOI: 10.1111/bph.15895
    Sample: plasma,brain
  3. JOURNAL OF CONTROLLED RELEASE (2020) IF: 7.727
    Photo-responsive supramolecular hyaluronic acid hydrogels for accelerated wound healing

    DOI: 10.1016/j.jconrel.2020.04.014

    PMID: 32283209

    		 Sample: Tissue homogenate
  4. Frontiers in Cellular and Infection Microbiology (2022) IF: 6.073
    Liujunanwei decoction attenuates cisplatin-induced nausea and vomiting in a Rat-Pica model partially mediated by modulating the gut micsrobiome

    DOI: 10.3389/fcimb.2022.876781

    PMID: 36061858

    		 Sample: jejunum tissue,medulla oblongata tissue
  5. Biomedicines (2022) IF: 6.081
    The Role of Neuregulin-1 in Steatotic and Non-Steatotic Liver Transplantation from Brain-Dead Donors

    DOI: 10.3390/biomedicines10050978

    PMID: 35625715

    		 Sample: liver tissue
  6. American Journal of Cancer Research (2021) IF: 6.166
    Adamantane-linked isothiourea derivatives suppress the growth of experimental hepatocellular carcinoma via inhibition of TLR4-MyD88-NF-κB signaling

    PMID: 33575076

    		 Sample: Tissue homogenate(liver tissue),Serum
  7. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    L-ergothioneine and its combination with metformin attenuates renal dysfunction in type-2 diabetic rat model by activating Nrf2 antioxidant pathway

    DOI: 10.1016/j.biopha.2021.111921

    PMID: 34346315

    		 Sample: kidney
  8. LIFE SCIENCES (2022) IF: 6.78
    Femtosecond laser attenuates oxidative stress, inflammation, and liver fibrosis in rats: Possible role of PPARγ and Nrf2/HO-1 signaling

    DOI: 10.1016/j.lfs.2022.120877

    PMID: 35963297

    		 Sample: liver
  9. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021) IF: 6.953
    Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment

    DOI: 10.1016/j.ijbiomac.2021.05.188

    PMID: 34062160

    		 Sample: Serum
  10. BIOMACROMOLECULES (2021) IF: 6.988
    Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model

    DOI: 10.1021/acs.biomac.0c01709

    PMID: 33899465

    		 Sample: Tissue homogenate,Serum
  11. Frontiers in Medicine (2022) IF: 5.058
    Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

    DOI: 10.3389/fmed.2022.953749
    Sample: renal tissue
  12. Frontiers in Medicine (2021) IF: 5.093
    Oxymatrine Protects TGFβ1-Induced Retinal Fibrosis in an Animal Model of Glaucoma.

    DOI: 10.3389/fmed.2021.750342
    Sample: Retinal Layers
  13. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
    Mesenchymal stem cells ameliorate silica-induced pulmonary fibrosis by inhibition of inflammation and epithelial-mesenchymal transition

    DOI: 10.1111/jcmm.16621

    PMID: 34076355

    		 Sample: lung,bronchoalveolar lavage fluid
  14. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    		 Sample: serum
  15. RESPIRATORY RESEARCH (2021) IF: 5.631
    Artesunate ameliorates cigarette smoke-induced airway remodelling via PPAR-γ/TGF-β1/Smad2/3 signalling pathway

    DOI: 10.1186/s12931-021-01687-y

    PMID: 33757521

    		 Sample: BALF
  16. Aging-US (2021) IF: 5.682
    Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation

    DOI: 10.18632/aging.202399

    PMID: 33495412

    		 Sample: Serum,Cell culture supernatant
  17. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

    DOI: 10.1002/tox.23464

    PMID: 35006638

    		 Sample: bronchoalveolar lavage fluid (BALF)
  18. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Imbalanced inflammatory response in subchronic arsenic-induced liver injury and the protective effects of Ginkgo biloba extract in rats: Potential role of cytokines mediated cell–cell interactions

    DOI: 10.1002/tox.23324
    Sample: serum,liver
  19. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY (2022) IF: 4.369
    BMSC-EVs overexpressing PD-L1 ameliorate DSS-induced ulcerative colitis in rats by regulating Th17/Treg cell balance through PTEN/PI3K/AKT/mTOR axis

    DOI: 10.1111/jgh.15987
    Sample: blood,colon
  20. INFLAMMOPHARMACOLOGY (2022) IF: 4.473
    Anti-inflammatory potential of liraglutide, a glucagon-like peptide-1 receptor agonist, in rats with peripheral acute inflammation

    DOI: 10.1007/s10787-022-00978-0

    PMID: 35412166

    		 Sample: paw tissue
  21. Aging-US (2020) IF: 4.831
    Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure

    DOI: 10.18632/aging.103827

    PMID: 33159018

    		 Sample: Cell lysate
  22. PLANTA MEDICA (2022) IF: 3.007
    Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats

    DOI: 10.1055/a-1997-5566
    Sample: burn wound tissue
  23. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Assessment of the protective and therapeutic effect of melatonin against thioacetamide‐induced acute liver damage

    DOI: 10.1002/jbt.22450
    Sample: Tissue homogenate
  24. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.652
    Polydatin combats methotrexate-induced pulmonary fibrosis in rats: Involvement of biochemical and histopathological assessment

    DOI: 10.1002/jbt.23019
    Sample: lung
  25. JOURNAL OF KING SAUD UNIVERSITY SCIENCE (2022) IF: 3.829
    Polyherbal formulation: The studies towards identification of composition and their biological activities

    DOI: 10.1016/j.jksus.2022.102256
    Sample: serum
  26. PHARMACEUTICAL BIOLOGY (2023) IF: 3.889
    Nanocurcumin preserves kidney function and haematology parameters in DMBA-induced ovarian cancer treated with cisplatin via its antioxidative and anti-inflammatory effect in rats

    DOI: 10.1080/13880209.2023.2166965
    Sample: plasma
  27. Annals of Translational Medicine (2022) IF: 3.932
    MMP2/9 downregulation is responsible for hepatic function recovery in cirrhotic rats following associating liver partition and portal vein ligation for staged hepatectomy

    DOI: 10.21037/atm-22-1312
    Sample: serum
  28. NEUROTOXICITY RESEARCH (2022) IF: 3.978
    Therapeutic and Preventive Effects of Piperine and its Combination with Curcumin as a Bioenhancer Against Aluminum-Induced Damage in the Astrocyte Cells

    DOI: 10.1007/s12640-022-00600-9
    Sample: primary astrocyte cell
  29. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Effects of vitamin B12 on methotrexate hepatotoxicity: evaluation of receptor-interacting protein (RIP) kinase

    DOI: 10.1007/s00210-020-01992-1

    PMID: 33052426

    		 Sample: Tissue homogenate
  30. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2020) IF: 2.107
    The dynamic expression of canonical Wnt/β-catenin signalling pathway in the pathologic process of experimental autoimmune neuritis

    DOI: 10.1080/00207454.2020.1725511
    Sample: sciatic nerves
  31. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Aberrant effect of genistein on placenta development expressed through alteration in transforming growth factor-β1 and alkaline phosphatase across the maternal serum, the placenta and the amniotic fluid

    DOI: 10.22038/ijbms.2020.42493.10022

    PMID: 33149862

    		 Sample: Serum
  32. Pharmacological Reports (2016) IF: 2.251
    The role of histamine in the regulation of the viability, proliferation and transforming growth factor β1 secretion of rat wound fibroblasts

    DOI: 10.1016/j.pharep.2016.11.006
    Sample: fibroblasts
  33. Clinical Oral Investigations (2019) IF: 2.453
    Systemic and local effects of radiotherapy: an experimental study on implants placed in rats

    DOI: 10.1007/s00784-019-02946-5

    PMID: 31154539

    		 Sample: Serum
  34. Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
    L-Carnitine ameliorates the liver by regulating alpha-SMA, iNOS, HSP90, HIF-1alpha, and RIP1 expressions of CCL4-toxic rats

    DOI: 10.22038/IJBMS.2020.47711.10990

    PMID: 33953857

    		 Sample: Serum
  35. ANDROLOGIA (2021) IF: 2.775
    Protective effect of oltipraz in testicular ischaemia/reperfusion injury: An experimental study

    DOI: 10.1111/and.14245

    PMID: 34514606

    		 Sample: Testicular tissue
  36. Annals of Transplantation (2021) IF: 1.53
    Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia

    DOI: 10.12659/AOT.928047

    PMID: 33414361

    		 Sample: The amniotic fluid samples
  37. European Journal of Trauma and Emergency Surgery (2019) IF: 1.781
    Beneficial effects of bardoxolone methyl, an Nrf2 activator, on crush-related acute kidney injury in rats

    DOI: 10.1007/s00068-019-01216-z

    PMID: 31471671

    		 Sample: Tissue homogenate
  38. Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879
    Improvement in post-partum uterine involution in rats treated with Apios americana

    DOI: 10.1631/jzus.B1800475
    Sample: serum
  39. Journal of Diabetes and Metabolic Disorders (2022)
    Effect of Costus pictus per se and in combination with Metformin and Enalapril in streptozotocin induced diabetic nephropathy in rats

    DOI: 10.1007/s40200-022-01065-5

    PMID: 36404856

    		 Sample: serum
  40. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020) IF: 0.373
    Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions

    DOI: 10.1515/tjb-2019-0309
    Sample: serum
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