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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Cat.No.:E-BC-K261-S
Detection instrument: Spectrophotometer(510 nm)

Detection method: Colorimetric method

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Detection principle

The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.

Performance characteristics

Synonyms	TG
Sample type	Serum,plasma,tissue
Sensitivity	0.19 mmol/L
Detection range	0.19-8.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 6.700%Average intra-assay CV: 2.400%
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.19-8.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Rat plasma	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Mouse brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) for serum (plasma) samples; The diluent is anhydrous ethanol for tissue samples.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

mBio (2021) IF: 7.867

Ileal Bile Acid Transporter Inhibitor Improves Hepatic Steatosis by Ameliorating Gut Microbiota Dysbiosis in NAFLD Model Mice | mBio

DOI: 10.1128/mBio.01155-21
	Sample: serum

Frontiers in Pharmacology (2018) IF: 5.811

Astragaloside IV Inhibits Triglyceride Accumulation in Insulin-Resistant HepG2 Cells via AMPK-Induced SREBP-1c Phosphorylation

DOI: 10.3389/fphar.2018.00345
	Sample: HepG2 cells

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