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Tryptophan (Trp) Fluorometric Assay Kit

  • Cat.No.:E-BC-F091

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/587 nm)

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  • 96T
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Detection principle

Tryptophan (Trp) is an essential amino acid involved in a variety of biosynthetic processes. It is not only involved in the synthesis of a variety of proteins, but also the precursor of many bioactive substances. The enzyme catalyzes Trp in a series of reactions to produce hydrogen peroxide, which forms a red complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured to calculate the content of Trp in the sample.

Performance characteristics

Synonyms Trp
Sample type serum;plasma;tissue;cell
Sensitivity 0.33 μmol/L
Detection range 0.33-200.00 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 2.5-5.6%Average intra-assay CV: 3.4-4.7%
Other instruments required Incubator(37℃), 3 KD ultrafiltration tube
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 12 months

Ralated product

Cat Number Product Name
E-BC-K852-M γ-Aminobutyric Acid (GABA) Colorimetric Assay Kit

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