ZO-1 Monoclonal Antibody

  • monoclonal antibody-Elabscience
    • monoclonal antibody-Elabscience
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    • monoclonal antibody-Elabscience
    • monoclonal antibody-Elabscience
      Catalog number:E-AB-27827

      Synonyms:DKFZp686M05161,Tight junction protein 1,Tight junction protein ZO 1,TJP1,ZO 1,ZO1,ZO-1,Zona occludens protein 1,Zonula occludens protein 1

      Size:
      • 120μL
      • 200μL
      • 60μL
      Qty:
      - +
      Price: $296

      Host: Mouse

      Reactivity: H

      Applications: ELISA,WB,IHC

      Lead Time: 7~10 days

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      Overview

      Synonyms DKFZp686M05161,Tight junction protein 1,Tight junction protein ZO 1,TJP1,ZO 1,ZO1,ZO-1,Zona occludens protein 1,Zonula occludens protein 1
      Source Mouse
      Reactivity Human
      Immunogen Fusion protein of ZO-1
      Application ELISA,WB,IHC
      Clonality Monoclonal

      Properties

      Clone No Clone:1G4A1
      Isotype IgG1
      Purification Protein G purification
      Conjugation Unconjugated
      Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
      Storage buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
      Recommended dilution WB:1:1000-1:4000, IHC:1:200-1:800
      Background Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells,which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium . ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells . It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin . ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation .

      Images

      HepG2 cells were subjected to SDS PAGE followed by western blot with ZO-1 Monoclonal Antibody at dilution of 1:2000.

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