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FITC Anti-Mouse/Rat Foxp3 Antibody[FJK-16s]

Uniprot : Q99JB6
  • Cat.No.:E-AB-F1351UC

  • Applications: ICFCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1351UC

Size:
  • 25μg
  • 100μg
Price: $140
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Antigen Details

Alternate Names Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Swissprot Q99JB6
Gene ID 20371
References
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.

Product Details

Form Liquid
Clone No. FJK-16s(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C]
Reactivity Mouse, Rat
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

Foxp3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of Foxp3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. Foxp3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of Foxp3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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