Click here to view cell apoptosis assay products
×

PE/Elab Fluor® 594 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s]

Uniprot : Q99JB6
  • Cat.No.:E-AB-F1351P

  • Applications: ICFCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1351P

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $160
Qty:

Antigen Details

Alternate Names Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Swissprot Q99JB6
Gene ID 20371
References
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.

Product Details

Form Liquid
Clone No. FJK-16s(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control PE/Elab Fluor® 594 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832P]
Reactivity Mouse, Rat
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Elab Fluor® 594
PE/Elab Fluor® 594 is designed to be excited by the blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 620 nm (e.g., a 610/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

Foxp3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of Foxp3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. Foxp3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of Foxp3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for 10 Test Free Trial FCM Antibody
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: